Hemoglobin Brockton [beta 138 (H16) Ala—-Professional] has reduced oxygen affinity but electrophoretic mobility and hemolysate binding are normal, suggesting that the substitution at beta 138 would not disrupt crucial polar interactions, including the hydrogen bonds and salt bridges amongst Asp 137 and Pro 138, that contribute to your cooperative oxygen-binding https://nicolaslecourtmansion.com